[1]张力文,王兴桂,李春,等.苗药组方熏蒸疗法对家兔早期膝骨性关节炎模型的差异基因分析及聚类分析[J].西部中医药,2024,37(02):20-26.[doi:10.12174/j.issn.2096-9600.2024.02.05]
　ZHANG Liwen,WANG Xinggui,LI Chun,et al.Cluster Analysis of Differentially Expressed Genes of Rabbit Models of Early Knee Osteoarthritis Treated by Fumigation Therapy of Miao Ethnic Group Prescriptions[J].Western Journal of Traditional Chinese Medicine,2024,37(02):20-26.[doi:10.12174/j.issn.2096-9600.2024.02.05]
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苗药组方熏蒸疗法对家兔早期膝骨性关节炎模型的差异基因分析及聚类分析
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
37
期数:
2024年02期
页码:
20-26
栏目:
基础研究
出版日期:
2024-02-15

文章信息/Info

Title:
Cluster Analysis of Differentially Expressed Genes of Rabbit Models of Early Knee Osteoarthritis Treated by Fumigation Therapy of Miao Ethnic Group Prescriptions
作者:
张力文, 王兴桂, 李春, 庞平
贵州中医药大学，贵州 花溪 550025
Author(s):
ZHANG Liwen, WANG Xinggui, LI Chun, PANG Ping
Guizhou University of Traditional Chinese Medicine, Huaxi 550025, China
关键词:
骨性关节炎; 膝; 基因芯片技术; 苗药熏蒸疗法; 差异表达; 信号通路
Keywords:
osteoarthritis;  knee; gene chip technology; fumigation therapy of ethnic group prescriptions; differentially expressed genes; signal pathway
分类号:
R684.3
DOI:
10.12174/j.issn.2096-9600.2024.02.05
文献标志码:
A
摘要:
目的探讨苗药组方熏蒸疗法对家兔早期膝骨性关节炎（knee osteoarthriti，KOA）模型差异表达基因进行层次聚类分析的意义。 方法将新西兰兔40只随机分为4组，空白对照组、模型对照组、苗药熏蒸组、清水对照组，每组10只。采用木瓜蛋白酶在兔膝关节腔内注射的方法复制早期KOA模型，造模成功后用苗药熏蒸疗法治疗20天，运用基因芯片检测技术对软骨组织的基因表达谱进行检测，最后将得到的原始数据通过RMA算法进行预处理，通过相关基因分析软件进行差异基因（difference gene，DEG）分析，并对其基因表达谱进行聚类分析。 结果DEG分析显示，模型对照组与空白对照组比较，差异显著基因有827个，上调435个，下调392个；苗药熏蒸组与模型对照组比较，差异显著基因有208个，上调112个，下调96个；清水熏蒸组与模型对照组比较，差异显著基因有152个，上调93个，下调59个。聚类分析显示，每个实验组的标本基本为一类，每个标本重复性好，每个实验组与对照组比较，它们的基因表达各有特点。 结论基因芯片检测可以发现苗药熏蒸疗法治疗早期KOA的差异表达基因，而聚类分析发现其相关基因的改变及其基因表达的特殊性。
Abstract:
ObjectiveTo survey the significance of hierarchical cluster analysis of differentially expressed genes (DEG) in rabbit models of early knee osteoarthritis by fumigation therapy of Miao ethnic group prescrip-tions. MethodsForty New Zealand rabbits were randomized into four groups: blank control group, model control group, fumigation therapy group of Miao ethnic group prescriptions and fresh water control group, with ten in each group. Early KOA models were duplicated by injecting papain into the rabbit knee cavity, after successfully modeling, they were treated by fumigation therapy of Miao ethnic group prescriptions for 20 days, gene chip technology was used to perform the analysis of DEGs, consequently, to preprocess raw data obtained with RMA algorithm, and to perform cluster analysis of the gene expression spectrum. ResultsThe analysis of DEG showed that, when model control group was compared with blank control group, there were 827 DEGs, 435 up-regulated genes and 392 down-regulated ones; when fumigation therapy group of Miao ethnic group prescriptions was compared with model control group, there were 208 DEGs, 112 up-regulated ones and 96 down-regulated ones; when fresh water control group was compared with model control group, there were 152 DEGs, 93 up-regulated ones and 59 down-regulated ones. Cluster analysis displayed that the samples in each experiment group were basically the same type, and each sample showed good repeatability, genetic expression of each experimental group has its own characteristics when the experiment group was compared with the control group. ConclusionGene chip testing could discover DEGs of fumigation therapy of Miao ethnic group prescriptions for early KOA, while cluster analysis could find the changes of the related genes and the characteristics of genetic expression.

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备注/Memo

备注/Memo:
张力文（1987—），女，硕士学位，医师。研究方向：针灸推拿临床、科研及教学研究。国家自然科学基金（81260575）；贵州省科技厅一般项目（黔科合基础〔2021〕一般503）；贵州省卫生健康委科学技术基金项目（gzwkj2023-188）。
更新日期/Last Update: 2024-02-15