[1]薛建军,呼荟茹,齐兵献,等.参附注射液对心肌缺血再灌注老年大鼠NF-κB及IκB-α表达的影响*[J].西部中医药,2013,26(02):18-21.
　XUE Jianjun,HU Huiru,QI Bingxian,et al.Effects of ShenFu Injection on Expressions of NF-κB and IκB-α in Aged Rats with Myocardial Ischemia and Reperfusion[J].Western Journal of Traditional Chinese Medicine,2013,26(02):18-21.
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参附注射液对心肌缺血再灌注老年大鼠NF-κB及IκB-α表达的影响*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年02期
页码:
18-21
栏目:
出版日期:
2013-02-15

文章信息/Info

Title:
Effects of ShenFu Injection on Expressions of NF-κB and IκB-α in Aged Rats with Myocardial Ischemia and Reperfusion
文章编号:
1004-6852(2013)02-0018-04
作者:
薛建军1，呼荟茹2，齐兵献2，张凌云1，贾国龙1
1 甘肃省中医院，甘肃 兰州 730050；2 甘肃中医学院
Author(s):
XUE Jianjun1, HU Huiru2, QI Bingxian2, ZHANG Lingyun1, JIA Guolong1
1 Gansu Province Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu University of Traditional Chinese Medicine
关键词:
心肌缺血再灌注; 参附注射液; NF-κB; IκB-α
Keywords:
myocardial ischemia and reperfusion;  ShenFu injection;  NF-κB;  IκB-α
分类号:
R-332
文献标志码:
A
摘要:
目的：探讨参附注射液对心肌缺血再灌注（I/R）老年大鼠心肌组织NF-κB及IκB-α的影响。方法：健康Wistar老年大鼠30只，18月龄，随机分为假手术组、模型组、参附组，每组10只。采用结扎左冠状动脉前降支法制备I/R模型。运用免疫组化方法观察I/R后心肌组织细胞中NF-κB及IκB-α蛋白表达；通过电镜观察心肌组织细胞结构变化情况。结果：与假手术组比较，模型组NF-κB表达明显增加(P＜0.05)，IκB-α表达明显下降(P＜0.05)；与模型组相比，参附组NF-κB表达明显下降(P＜0.05)，IκB-α表达明显增加(P＜0.05)。电镜观察结果显示参附组心肌组织细胞结构受损明显减轻。结论：参附注射液可增加心肌组织中IκB-琢的水平，减弱其降解作用，通过抑制NF-κB活性，从而阻断NF-κB调控的炎症反应及细胞凋亡，对I/R后心肌组织损伤起防治作用。
Abstract:
Objective: To explore the effects of ShenFu injection on NF-κB and IκB-α in aged rats with myocardial ischemia and reperfusion (I/R). Method: Thirty aged rats were randomly divided into sham operation group (S group), model group (I/R group) and ShenFu group (SF group), ten rats each group. I/R models were established through ligation method of the left anterior descending coronary artery. The expressions of NF-κB and IκB-α in the cells of myocardial tissue were observed after I/R with immunohistochemical method; changes of cellular structure of myocardial tissue were explored with electron microscope. Result: Compared with S group,expressions of NF-κB in I/R group increased obviously (P＜0.05), expressions of IκB-α decreased notably (P＜0.05); compared with I/R group, the expressions of NF-κB in SF group lowered obviously(P＜0.05), expressions ofIκB-α increased notably(P＜0.05). The results of electron microscope revealed that the damage of cellular structurein the myocardial tissue of SF group was alleviated. Conclusion: ShenFu Injection could increase the level of IκB-α in the myocardial tissue, weaken its degradation, interrupt inflammatory reaction and cellular apoptosis regulated by NF-κB through inhibiting the activity of NF-κB and prevent the damage of myocardial tissue after I/R.

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备注/Memo

备注/Memo:
收稿日期：2012-09-20 * 基金项目：甘肃省自然科学研究基金(编号 1010RJZA209) 作者简介：薛建军(1971—)，男，硕士学位，副主任医师。研究方向：围手术期的器官保护。
更新日期/Last Update: 2013-02-15