[1]王保平,张圆,陈怡名,等.龙首参葛丸对血管性痴呆大鼠海马CA1区组织结构变化的影响[J].西部中医药,2019,32(06):6-9.
　WANG Baoping,ZHANG Yuan,CHEN Yiming,et al.Impact of LongShou ShenGe Pills on the Structural Changes of the Tissue in Hippocampal CAI Region of the Rat with Vascular Dementia[J].Western Journal of Traditional Chinese Medicine,2019,32(06):6-9.
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龙首参葛丸对血管性痴呆大鼠海马CA1区组织结构变化的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
32
期数:
2019年06期
页码:
6-9
栏目:
方药·药理研究
出版日期:
2019-06-15

文章信息/Info

Title:
Impact of LongShou ShenGe Pills on the Structural Changes of the Tissue in Hippocampal CAI Region of the Rat with Vascular Dementia
文章编号:
1004-6852(2019)06-0006-04
作者:
王保平1; 张圆2; 陈怡名1; 徐克国1; 马献中1
1 甘肃医学院，甘肃 平凉 744000； 2 甘肃医学院附属医院
Author(s):
WANG Baoping 1;  ZHANG Yuan2;  CHEN Yiming 1;  XU Keguo 1;  MA Xianzhong 1
1 Gansu Medical College, Pingliang 744000, China; 2 Affiliated Hospital of Gansu Medical College
关键词:
龙首参葛丸; 血管性痴呆; 大鼠; 海马CA1区组织结构; 动物实验
Keywords:
LongShou ShenGe pills;  vascular dementia;  rats;  the structure of the tissue in hippocampal CA1 region;  animal experiments
分类号:
R285.5
文献标志码:
A
摘要:
目的：观察龙首参葛丸对血管性痴呆大鼠海马CA1区组织结构变化的影响。方法：采用改良2-VO法复制血管性痴呆模型大鼠60只，分为正常组、阳性对照组(NS，10 mL/kg)，脑复康组(0.8 g/kg)，龙首参葛丸低剂量(1.5 g/kg)组、中剂量(3 g/kg)组、高剂量(6 g/kg)组，每组10只。经口灌胃等体积给药，10 mL/kg动物体质量，1次/日。在大鼠造模前及造模后第38天、39天、40天进行跳台试验，对大鼠进行学习记忆能力的评估。大鼠于第41 天给予10%水合氯醛麻醉迅速断头取脑，取视交叉后4 mm与小脑前之间的部分，经4%多聚甲醛浸置后，制作成厚约5 μm的冠状切片，HE染色后镜下观察海马CA1区组织结构变化。结果：跳台试验显示：龙首参葛丸低、中、高剂量组及脑复康组与阳性对照组比较，差异均有统计学意义(P＜0.05)，说明龙首参葛丸和脑复康都有改善大鼠学习记忆功能的作用，但龙首参葛丸高剂量组相较脑复康组作用更加明显(P＜0.05)；镜下染色观察：龙首葛根组与脑复康组、阳性对照组比较，大鼠海马区CA1区尼氏体(虎斑样结构)体积大、数量多，且分布较均匀，发生尼氏体溶解的细胞数量也较少。结论：龙首参葛丸可促进神经元内蛋白质合成，减少神经元损伤，促进神经元修复，对血管性痴呆具有治疗和保护作用。
Abstract:
Objective: To observe the effects of LongShou ShenGe pills on the structural changes of the tissue in hippocampal CAI region in the rats with vascular dementia (VD). Methods: sixtyVD rat models were duplicated using modified 2-VO method, and they were randomized into the normal group, positive control group (NS, 10 mL/mg), the group of NaoFuKang (0.8 g/kg), low (1.5 g/kg), moderate (3 g/kg) and high (6 g/kg) doses groups of LongShou ShenGe pills, oral gavage in equal volume, 10 mL/kg, body mass, once each day. Step down test was performed before modeling, at the 38 d, 39 d and 40 d after modeling to assess learning and memory ability. The rats were sacrificed at the 41st day after giving 10% chloral hydrate, the brain was drawn and the part 4mm behind chiasma opticum and before the epencephalon were taken, after managed with 4% paraformaldehyde, it was prepared into coronal section, around 5 μm thick, the slices were observed after HE staining. Results: Step down test showed that: the difference showed significant meaning when low, moderate and high doses groups of LongShou ShenGe pills, and NaoFuKang group were compared with positive control group (P＜0.05), which demonstrated that LongShou ShenGe pills and NaoFuKang could improve learning and memory ability of the rats, while the effects of high dose group of LongShou ShenGe pills were more notable compared with NaoFuKang group (P＜0.05). Observation under microscope after staining: When LongShou GeGen pill group was compared with NaoFuKang group and positive control group, the tigroid body in hippocampal CAI region was in large size, numerous and well-distributed, and the numbers of the cells appearing chromatolysis were fewer. Conclusion: LongShou ShenGe pill could promote protein synthesis in nerve cells, reduce neuron injury and improve the neuron repair, and it shows therapeutic effects and protective effects on VD.

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备注/Memo

备注/Memo:
收稿日期：2017-11-19 *基金项目：甘肃省中医药管理局科研课题(编号GZK-2015-37)。 作者简介：王保平(1979—)，男，副教授。研究方向：生物化学与分子生物学研究。
更新日期/Last Update: 2019-06-15