[1]邢亚东,武敏,胡轲,等.HPLC法测定舒乐康洗液中4种药效成分含量[J].西部中医药,2023,36(07):39-42.[doi:10.12174/j.issn.2096-9600.2023.07.08]
　XING Yadong,WU Min,HU Ke,et al.Simultaneous Determination of Four Kinds of Active Ingredients in Shulekang Lotion By HPLC[J].Western Journal of Traditional Chinese Medicine,2023,36(07):39-42.[doi:10.12174/j.issn.2096-9600.2023.07.08]
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HPLC法测定舒乐康洗液中4种药效成分含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
36
期数:
2023年07期
页码:
39-42
栏目:
出版日期:
2023-07-15

文章信息/Info

Title:
Simultaneous Determination of Four Kinds of Active Ingredients in Shulekang Lotion By HPLC
作者:
邢亚东1, 武敏2, 胡轲3, 孙大伟4, 李姗姗1
1.蚌埠医学院，安徽 蚌埠 233000
2.宿州市第一人民医院，安徽 宿州 234000
3.太和县食品药品检验所，安徽 太和 236600
4.安徽金太阳生化药业有限公司，安徽 颍上 236000
Author(s):
XING Yadong1, WU Min2, HU Ke3, SUN Dawei4, LI Shanshan1
1.Bengbu Medical College, Bengbu 233000, China
2.Suzhou First People's Hospital, Suzhou 234000, China
3.Taihe Institute for Food and Drug Control, Taihe 236600, China
4.Anhui Golden Sun Biochemical Pharmaceutical Co., LTD, Yingshang 236000, China
关键词:
高效液相色谱法; 舒乐康洗液; 咖啡酸; 苍术素; 盐酸小檗碱; 蛇床子素; 含量测定
Keywords:
HPLC; lotion; caffeic acid; atrctylodin; berberine hydrochloride; osthole; content determination
分类号:
R932
DOI:
10.12174/j.issn.2096-9600.2023.07.08
文献标志码:
A
摘要:
目的建立舒乐康洗液中苍术素、盐酸小檗碱、蛇床子素、咖啡酸含量测定的高效液相色谱方法。 方法Syncronis C18（250 mm×4.5 mm，4.6 μm），检测波长：323 nm；乙腈（A）-0.02 moL/L磷酸二氢钾溶液（含0.2%三乙胺，磷酸溶液调pH值至2.83）（B）；梯度洗脱；柱温：40 ℃；流速：1.0 mL/min。 结果苍术素、咖啡酸、盐酸小檗碱、蛇床子素分别在3.2317～48.4750 μg（r=0.9998）、0.6474～9.7110 μg（r=0.9996）、18.4200～276.3000 μg（r=0.9998）和6.1467～92.2000 μg（r=0.9997）范围内线性关系良好，平均加样回收率分别为99.37%、100.78%、99.48%和98.98%，RSD分别为1.09%、1.31%、0.97%和0.89%。 结论该法操作简便、稳定、重现性良好，结果准确，适合用于舒乐康洗液中苍术素、盐酸小檗碱、蛇床子素、咖啡酸含量分析。
Abstract:
ObjectiveTo establish a method of determining the contents of atrctylodin, berberine hydrochloride, osthole and caffeic acid contained in Shulekang lotion by HPLC. MethodsSyncronis C18 (250 mm×4.5 mm, 4.6 μm), detection wavelength: 323nm; acetonitrile - 0.02mol/L potassium dihydrogen phosphate solution (containing 0.2% triethylamine, adjust the pH value of the phosphate acid solution to 2.83); gradient elution; column temperature: 40 ℃; current velocity: 1.0 mL/min. ResultsAtrctylodin, berberine hydrochloride, osthole and caffeic acid presented good linear relation in the range between 3.2317 and 48.4750 μg (r=0.9998), 0.6474 and 9.7110 μg (r=0.9996), 18.4200 and 276.3000 μg (r=0.9998), 6.1467 and 92.2000 μg (r=0.9997) respectively, average sample recovery rates were 99.37%, 100.78%, 99.48% and 98.98% respectively, with RSD of 1.09%, 1.31%, 0.97% and 0.89%. ConclusionThe method, simple and convenient, stable, reproducible and accurate, is suitable for the content analysis of atrctylodin, berberine hydrochloride, osthole and caffeic acid contained in Shulekang lotion.

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备注/Memo

备注/Memo:
邢亚东（1987—），男，硕士学位，主管药师。研究方向：中药制剂及中药质量控制与评价。安徽省自然科学基金（1908085QH373）；蚌埠医学院自然科学基金面上项目（BYKY1873）。
更新日期/Last Update: 2023-07-15