[1]陈财,曾平,刘金富,等.基于网络药理学探讨薏苡仁汤治疗膝骨关节炎的作用机制[J].西部中医药,2025,38(05):31-37.[doi:10.12174/j.issn.2096-9600.2025.05.08]
　CHEN Cai,ZENG Ping,LIU Jinfu,et al.Network Pharmacology-based Exploration into the Treatment of Knee Osteoarthritis with Coix Seed Decoction[J].Western Journal of Traditional Chinese Medicine,2025,38(05):31-37.[doi:10.12174/j.issn.2096-9600.2025.05.08]
点击复制

基于网络药理学探讨薏苡仁汤治疗膝骨关节炎的作用机制()

《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
38
期数:
2025年05期
页码:
31-37
栏目:
二次研究
出版日期:
2025-05-15

文章信息/Info

Title:
Network Pharmacology-based Exploration into the Treatment of Knee Osteoarthritis with Coix Seed Decoction
作者:
陈财1, 曾平2, 刘金富2, 钱晓芬1
1.广西中医药大学研究生院，广西 南宁 530299
2.广西中医药大学第一附属医院，广西 南宁 530023
Author(s):
CHEN Cai1, ZENG Ping2, LIU Jinfu2, QIAN Xiaofen1
1.Graduate School of Guangxi University of Chinese Medicine, Nanning 530299, China
2.The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
关键词:
膝; 骨关节炎; 网络药理学; 薏苡仁汤; 作用机制
Keywords:
knee;  osteoarthritis; network pharmacology; coix seed decoction; mechanism
分类号:
R255.6
DOI:
10.12174/j.issn.2096-9600.2025.05.08
文献标志码:
A
摘要:
目的运用网络药理学方法分析薏苡仁汤治疗膝骨关节炎的作用机制。 方法利用TCMSP数据库中的药动学参数，以口服生物利用度（oral bioavailability，OB）≥30%和药物相似性（drug-likeness，DL）≥0.18筛选薏仁汤的潜在有效成分和作用靶点。通过DrugBank、GeneCard、OMIM、TTD和PharmGkb数据库筛选膝骨关节炎靶基因；通过R语言软件Venn分析法得到疾病与药物共同靶基因。通过Cytoscape 3.8.0软件构建成分-靶点网络图。利用STRING平台构建蛋白-蛋白互作网络（protein-protein interactions，PPI），将得到的文本文件导入Cytoscape中，通过CytoNCA插件获取关键子网络模块。将上述交集基因通过R语言软件进行基因本体（gene ontology，GO）功能富集分析、京都基因和基因组百科全书（kyoto encyclopedia of genes and genomes，KEGG）通路富集分析。 结果共筛选出薏仁汤有效活性成分168个，包括谷甾醇、β-谷甾醇、香叶木素、汉黄芩素、槲皮素和豆甾醇等。有效作用靶点232个，药物疾病共同靶点129个，核心靶点包括JUN、MAPK3、STAT3、MAPK1、TP53、MAPK14、MYC、ESR1、MAPK8、AKT1、RELA、IL-6、TNF和EGFR。GO富集结果表明目标靶点参与的功能主要有细胞对化学应激的反应、对氧化应激的反应和对脂多糖的反应。KEGG主要富集在IL-17信号通路和TNF信号通路。 结论薏仁汤可能通过多成分、多靶点和多途径对膝骨关节炎发挥治疗作用。
Abstract:
ObjectiveTo analyze the mechanism of Yiyiren Tang (coix seed decoction) in the treatment of knee osteoarthritis (KOA) using network pharmacology. MethodsPharmacokinetic parameters of TCMSP was utilized to screen the potential active ingredients and the targets of action of the decoction with OB≥30% and DL≥0.18. DrugBank, GeneCard, OMIM, TTD and PharmGkb databases were applied to screen the genes of KOA targets; shared target genes of disease and medicine were obtained via Venn diagram of R language software. Component-target network was constructed via Cytoscape 3.8.0 software. STRING platform was used to build protein-protein interactions (PPI), the key subnetwork module was obtained via CytoNCA plug-in after the text file was introduced into Cytoscape. GO and KEGG enrichment analysis of the above intersected genes were performed via R language software. ResultsA total of 168 active ingredients of the medicine have been screened, including stigmasterol, β -stigmasterol, camphor lignin, baicalin, quercetin and daucosterol, involving 232 active ingredients and 129 shared targets between medicine and disease, and the core targets contained JUN, MAPK3, and STAT3, MAPK1, TP53, MAPK14, MYC, ESR1, MAPK8, AKT1, RELA, IL-6, TNF and EGFR. GO enrichment results revealed that the targets were mainly involved in the response of cells to chemical stress, oxidative stress and lipopolysaccharide. KEGG is mainly enriched in IL-17 signaling pathway and TNF signaling pathway. ConclusionThe decoction could treat KOA possibly through multiple ingredients, multiple targets and multiple pathways.

相似文献/References:

[1]李兴勇,徐霞,麻虎,等.中医药治疗膝关节骨性关节炎的现状[J].西部中医药,2013,26(12):144.
　LI Xingyong,XU Xia,MA Hu,et al.Progress of Treatment for Knee Osteoarthritis by TCM[J].Western Journal of Traditional Chinese Medicine,2013,26(05):144.
[2]周立亚,刘德玉,李福林,等.热敷散外敷治疗兔骨性关节炎的机理研究[J].西部中医药,2014,27(10):22.
[3]尤从新.樊成虎教授治疗膝关节骨性关节炎经验[J].西部中医药,2014,27(12):33.
[4]李春娥,董建文,王文波.膝关节骨性关节炎熏洗方用药规律文献研究[J].西部中医药,2015,28(03):97.
[5]王想福,石瑞芳,武纪玲,等.玻璃酸钠单独使用与联合解毒消瘀膏治疗膝骨性关节炎疗效观察[J].西部中医药,2015,28(03):118.
[6]周天恒.针灸治疗膝骨性关节炎临床观察[J].西部中医药,2015,28(04):123.
[7]王艳丽,庞颖.膝关节置换术的围手术期护理与预后分析[J].西部中医药,2013,26(06):117.
　WANG Yanli,PANG Ying.Perioperative Care and Prognosis of Knee Replacement[J].Western Journal of Traditional Chinese Medicine,2013,26(05):117.
[8]陆金金,欧阳八四.电针与温针灸治疗瘀血阻滞型膝骨关节炎的疗效比较[J].西部中医药,2014,27(04):119.
　LU Jinjin,OUYANG Basi.Comparison of Clinical Effects between Acusector and Warm Needling Moxibustion in Treating Knee Osteoarthritis of Blood Stasis Obstruction Pattern[J].Western Journal of Traditional Chinese Medicine,2014,27(05):119.
[9]田雪梅,张丽君,柳树英.中医外治法辨证治疗膝骨性关节炎120例[J].西部中医药,2012,25(10):95.
　TIAN Xue-mei,ZHANG Li-jun,LIU Shu-ying.Syndrome Differentiation of TCM External Treatment for 120 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2012,25(05):95.
[10]赵道洲,郑恒恒.手法配合陇中Ⅰ号汽雾透皮疗法治疗膝骨性关节炎的临床研究*[J].西部中医药,2013,26(04):36.
　ZHAO Daozhou,ZHENG Hengheng.Clinical Study on Manipulation and Gansu Mist Transdermal Therapy No.1 in Treating Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2013,26(05):36.
[11]张建林.中药熏洗联合关节内注射玻璃酸钠治疗膝骨关节炎30例疗效观察[J].西部中医药,2012,25(09):80.
　ZHANG Jian-lin.Curative Observation on Fumigation with Herb and Intra-Articular Sodium Hyaluronate Injection in Treating 30 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2012,25(05):80.
[12]王亚伟,张彦军.中西医综合治疗膝骨性关节炎疗效观察[J].西部中医药,2012,25(05):80.
　WANG Ya-wei,ZHANG Yan-jun.Observation on Comprehensive Treatment of Knee Osteoarthritis with Integrated Traditional Chinese and Western Medicine[J].Western Journal of Traditional Chinese Medicine,2012,25(05):80.
[13]王兴昌.小针刀治疗膝骨性关节炎50例[J].西部中医药,2017,30(08):139.
　WANG Xingchang.Small Needle-knife in Treating 50 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2017,30(05):139.
[14]李盛华,吴献毅,周明旺,等.细胞凋亡在膝骨性关节炎半月板损伤中的作用研究[J].西部中医药,2018,31(02):131.
　LI Shenghua,WU Xianyi,ZHOU Mingwang,et al.Research on the Roles of Cellular Apoptosis Played in Meniscus Injury of the Patients with Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2018,31(05):131.
[15]张孝悌,冯方,孙育良.关节镜清理术联合中药熏洗治疗膝关节骨关节炎疗效观察[J].西部中医药,2018,31(12):114.
　ZHANG Xiaoti,FENG Fang,SUN Yuliang.Observation on Clinical Effects of Arthroscopic Debridement Combined with Herbal Fumigation in the Treatment for Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2018,31(05):114.
[16]周建,汤喜红.度洛西汀联合通络治痹汤加减治疗膝骨性关节炎41例[J].西部中医药,2020,33(04):109.[doi:10.12174/j.issn.1004-6852.2020.04.33]
　ZHOU Jian,TANG Xihong.Duloxetine and Modified Tongluo Zhibi Tang in Treating 41 Cases of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2020,33(05):109.[doi:10.12174/j.issn.1004-6852.2020.04.33]
[17]刘亮,等.盘龙七片联合硫酸氨基葡萄糖胶囊治疗膝骨性关节炎的临床研究[J].西部中医药,2022,35(02):99.[doi:10.12174/j.issn.2096-9600.2022.02.24]
　LIU Liang,WU Jiafeng,et al.Clinical Study on Panlongqi Tablets and Glucosamine Sulfate Capsule in the Treatment of Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2022,35(05):99.[doi:10.12174/j.issn.2096-9600.2022.02.24]
[18]瞿龙,张桂香,王文哲.土藤草汤对膝骨关节炎临床疗效及炎性因子的影响[J].西部中医药,2022,35(04):106.[doi:10.12174/j.issn.2096-9600.2022.04.24]
　QU Long,ZHANG Guixiang,WANG Wenzhe.Clinical Effects of Tutengcao Decoction in the Treatment of Knee Osteoarthritis and Its Influence on Inflammatory Factor[J].Western Journal of Traditional Chinese Medicine,2022,35(05):106.[doi:10.12174/j.issn.2096-9600.2022.04.24]
[19]王杰,陈帅,唐望,等.消肿定痛合剂联合交联玻璃酸钠治疗气滞血瘀型膝骨关节炎的临床疗效观察[J].西部中医药,2022,35(08):104.[doi:10.12174/j.issn.2096-9600.2022.08.25]
　WANG Jie,CHEN Shuai,TANG Wang,et al.Clinical Effects of Xiaozhong Dingtong Mixture and Crosslinked Sodium Hyaluronate in the Treatment of Qi Stagnation and Blood Stasis Pattern Knee Osteoarthritis[J].Western Journal of Traditional Chinese Medicine,2022,35(05):104.[doi:10.12174/j.issn.2096-9600.2022.08.25]
[20]李粟玉,陈广峰,郝锦秋,等.针刀治疗膝骨关节炎的施术部位和机制研究[J].西部中医药,2022,35(12):163.[doi:10.12174/j.issn.2096-9600.2022.12.38]
　LI Suyu,CHEN Guangfeng,HAO Jinqiu,et al.Study on the Operation Sites of Treating Knee Osteoarthritis by Acupotomy and the Mechanism[J].Western Journal of Traditional Chinese Medicine,2022,35(05):163.[doi:10.12174/j.issn.2096-9600.2022.12.38]

备注/Memo

备注/Memo:
陈财（1994—），男，在读硕士研究生。研究方向：四肢骨病与创伤的防治研究。国家自然科学基金（81960876）；广西中医药适宜技术开发与推广项目（GZSY21-14）。
更新日期/Last Update: 2025-05-15