[1]刘其平,强生毅,周钢.吴紫糊中左旋紫草素的含量测定*[J].西部中医药,2014,27(08):14-16.
 LIU Qiping,QIANG Shengyi,ZHOU Gang.Content Determination of Shikonin in WuZi Cataplasm[J].Western Journal of Traditional Chinese Medicine,2014,27(08):14-16.
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吴紫糊中左旋紫草素的含量测定*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年08期
页码:
14-16
栏目:
方药·制剂工艺
出版日期:
2014-08-15

文章信息/Info

Title:
Content Determination of Shikonin in WuZi Cataplasm
文章编号:
1004-6852(2014)08-0014-03
作者:
刘其平强生毅周钢
白银市第二人民医院,甘肃 白银 730900
Author(s):
LIU Qiping QIANG Shengyi ZHOU Gang
Baiyin Municipality the Second People′s Hospital, Baiyin 730900, China
关键词:
吴紫糊高效液相色谱法左旋紫草素含量测定
Keywords:
WuZi cataplasm high performance liquid chromatography shikonin content determination
分类号:
R927.2
文献标志码:
A
摘要:
目的:建立吴紫糊中左旋紫草素的测定方法。方法:高效液相色谱法,色谱条件色谱柱为安捷伦C18柱(4.6 mm×250 mm,5 μm),流动相为甲醇-0.1%磷酸盐缓冲液(80∶20),检测波长516 nm,柱温25℃,流速10 mL/min。结果:进样量在8.2~41.0 μg/mL范围内,线性关系良好(r=0.999 9)平均回收率为101.30%(RSD为1.55%,n=9)。结论:高效液相色谱法测定吴紫糊中左旋紫草素含量结果准确、重现性好,适用于吴紫糊的质量控制。
Abstract:
Objective: To establish the determination method for shikonin in WuZi cataplasm. Methods: High performance liquid chromatography(HPLC), chromatographic condition, chromatographic column: agilent C18 column (4.6 mm×250 mm, 5 μm), mobile phase: methanol-0.1% phosphate buffer solution(80:20), detection wavelength: 516nm, column temperature:25℃, flow rate was 10mL/min. Results: Sample size was in the range between 8.2 and 41.0 μg/mL, linear relationship was better (r=0.999 9), average recovery rate was 101.30% (RSD was 1.55%, n=9). Conclusion: HPLC determining the contents of shikonin in WuZi cataplasm is accurate and reproducible, which could be applied to quality control of WuZi cataplasm.

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备注/Memo

备注/Memo:
收稿日期:2014-03-05*基金项目:白银市2010年第二批科技计划项目(编号 GK20103-2-059A)作者简介:刘其平(1974—),男,主管药师。研究方向:中药制剂的研究。
更新日期/Last Update: 2014-08-15