[1]陈二林,黄艳辉,刘效栓,等.养阴生肌散中龙胆苦苷的HPLC测定[J].西部中医药,2013,26(10):24-26.
　CHEN Erlin,HUANG Yanhui,LIU Xiaoshuan,et al.Determination of Gentiamarin in YangYin ShengJi Powder by HPLC[J].Western Journal of Traditional Chinese Medicine,2013,26(10):24-26.
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养阴生肌散中龙胆苦苷的HPLC测定
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
26
期数:
2013年10期
页码:
24-26
栏目:
出版日期:
2013-10-15

文章信息/Info

Title:
Determination of Gentiamarin in YangYin ShengJi Powder by HPLC
文章编号:
1004-6852(2013)10-0024-03
作者:
陈二林1，黄艳辉2，刘效栓1，李喜香1
1 甘肃省中医院，甘肃 兰州 730050； 2 甘肃省肿瘤医院
Author(s):
CHEN Erlin1, HUANG Yanhui2, LIU Xiaoshuan1, LI Xixiang1
1 Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China; 2 Gansu Province Cancer Hospital
关键词:
高效液相色谱法; 养阴生肌散; 龙胆苦苷
Keywords:
HPLC;  YangYin ShengJi powder;  gentiamarin
分类号:
R282
文献标志码:
A
摘要:
目的：建立养阴生肌散中龙胆苦苷的含量测定方法。方法：采用色谱柱为Waters Symmetry Shield-C18柱(4.6 mm×250 mm，5 μm)，流动相为乙腈-0.2%磷酸溶液(25∶75)；流速为1.0 mL/min，柱温为室温，检测波长为265 nm。结果：龙胆苦苷在0.52～2.60 μg范围内呈良好的线性关系，相关系数r=0.999 4，平均回收率为99.06%，RSD为1.85%(n=6)。结论：该测定方法简便快速、准确可靠，可用于该制剂的质量控制。
Abstract:
Objective: To establish the content determination method of gentiamarin in YangYin ShengJi powder. Method: Chromatographic column was Waters Symmetry Shield-C18 column(4.6 mm×250 mm, 5 μm), mobile phase was acentonitrile-0.2% phosphoric acid(25:75); the flow rate was 1.0mL/min, the column temperature was room temperature, detection wavelength was 265nm. Result: Gentiamarin showed a good linear relationship in the range between 0.52 and 2.60 μg, r=0.999 4, average recovery rate was 99.06%, RSD=1.85% (n=6). Conclusion: The determination method, simple, rapid, accurate and reliable, could be used for quality control of the preparation.

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备注/Memo

备注/Memo:
收稿日期：2013-03-18 作者简介：陈二林(1979—)，男，硕士学位，主管药师。研究方向：中药及制剂质量控制研究。
更新日期/Last Update: 2013-10-15