[1]姜华,郭敏,徐世红,等.HPLC法测定忍冬藤痛风颗粒中绿原酸的含量[J].西部中医药,2017,30(04):25-27.
　JIANG Hua,GUO Min,XU Shihong,et al.Determination of Chlorogenic Acid in RenDongTeng Gouty Granules by HPLC[J].Western Journal of Traditional Chinese Medicine,2017,30(04):25-27.
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HPLC法测定忍冬藤痛风颗粒中绿原酸的含量()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
30
期数:
2017年04期
页码:
25-27
栏目:
出版日期:
2017-04-15

文章信息/Info

Title:
Determination of Chlorogenic Acid in RenDongTeng Gouty Granules by HPLC
文章编号:
1004-6852(2017)04-0025-03
作者:
姜华1，郭敏2，徐世红 2，谢兴文1
1 甘肃省中医药研究院，甘肃 兰州 730050； 2 甘肃省中医院
Author(s):
JIANG Hua1, GUO Min2, XU Shihong2, XIE Xingwen1
1 Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China;2 Gansu Provincial Hospital of Traditional Chinese Medicine
关键词:
忍冬藤痛风颗粒; 高效液相色谱法; 绿原酸
Keywords:
RenDongTeng gouty granules;  HPLC;  chlorogenic acid
分类号:
R284.1
文献标志码:
A
摘要:
目的：建立忍冬藤痛风颗粒中绿原酸含量的测定方法。方法：采用高效液相色谱法，色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm，5 μm)，以乙腈-0.1%磷酸水溶液(10∶90)为流动相，流速为1.0 mL/min，检测波长327 nm，柱温30℃进行检测。结果：绿原酸的线性范围为：13.88～48.58 μg/mL，线性方程为：Y=41 313X+42 348，r=0.999 8；平均加样回收率为99.54%(RSD=0.87%)。结论：该法稳定、快速、简便，结果准确、可靠，可用于忍冬藤颗粒中绿原酸的含量测定。
Abstract:
Objective: To establish the determination method of the contents of chlorogenic acid in RenDongTeng gouty granules. Methods: HPLC method was adopted, chromatographic column was Agilent Eclipse Plus C18(250 mm×4.6 mm, 5 μm), acetonitrile-0.1% phosphoric acid aqueous solution(10:90) was taken as the mobile phase, velocity was 1.0 mL/min, detection wavelength was 327 nm, column temperature 30℃. Results: Linear range of chlorogenic acid was between 13.88 and 48.58 μg/mL ,linear equation was: Y=41 313 X+42 348, r=0.999 8; average recovery rate was 99.54% (RSD=0.87%). Conclusion: The method, stable, rapid, convenient, accurate and reliable, could be used for content determination of chlorogenic acid in RenDongTeng gouty granules.

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备注/Memo

备注/Memo:
收稿日期：2016-10-27 *基金项目：甘肃省战略性新兴产业创新支撑工程项目(编号)；兰州市科技计划项目(生物医药)(编号2013-4-86)。 作者简介：姜华(1959—)，女，博士学位，主任药师，研究员。研究方向：中药化学及质量分析。
更新日期/Last Update: 2017-04-15