[1]王红丽,展锐,郭敏.HPLC法测定射干扶正口服液中射干苷的含量[J].西部中医药,2018,31(04):35-36.
　WANG Hongli,ZHAN Rui,GUO Min.The Contents of Belamcandin in SheGan FuZheng Oral Liquid Determined by HPLC[J].Western Journal of Traditional Chinese Medicine,2018,31(04):35-36.
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HPLC法测定射干扶正口服液中射干苷的含量()
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年04期
页码:
35-36
栏目:
出版日期:
2018-04-15

文章信息/Info

Title:
The Contents of Belamcandin in SheGan FuZheng Oral Liquid Determined by HPLC
文章编号:
1004-6852(2018)04-0035-02
作者:
王红丽，展锐，郭敏
甘肃省中医院，甘肃 兰州 730050
Author(s):
WANG Hongli, ZHAN Rui, GUO Min
Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China
关键词:
射干扶正口服液; 高效液相色谱法; 射干苷
Keywords:
SheGan FuZheng oral liquid;  HPLC;  belamcandin
分类号:
R282
文献标志码:
A
摘要:
目的：建立射干扶正口服液中射干苷的含量测定方法。方法：采用高效液相色谱法(HPLC)，色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm，5 μm)，以乙腈-水（28:72）为流动相，流速为1.0 mL/min，检测波长265 nm，柱温30℃进行检测。结果：射干苷的线性范围为：18.40～184.00 μg/mL，线性方程为：Y=44.21X-0.6，r=0.999 8，r=0.999 8；平均加样回收率为99.75%（RSD%=1.11%）。结论：该法稳定、快速、简便，结果准确、可靠，可用于射干扶正口服液中射干苷的含量测定。
Abstract:
Objective: To establish the content determination method of belamcandin in SheGan FuZheng oral liquid. Methods: HPLC was adopted when chromatographic column was Agilent Eclipse Plus C18 (250 mm×4.6 mm, 5 μm), methyl cyanide-water (28∶72) was taken as mobile phase, flow rate: 1.0 mL/min, detection wavelength: 265 nm, column temperature: 30℃. Results: Linearity range of belamcandin was 18.40~184.00 μg/mL, linear equation was: Y=44.21X-0.6, r=0.999 8, r=0.999 8, average recovery rate was 99.75% (RSD%=1.11%). Conclusion: The method, stable, rapid, simple, accurate and reliable, could be used for content determination ofbelamcandin in SheGan FuZheng oral liquid.

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备注/Memo

备注/Memo:
收稿日期：2017-10-27 *基金项目：甘肃省中医药管理局课题(编号GZK-2014-65)。 作者简介：王红丽(1978—)，女，硕士学位，副主任药师。研究方向：天然产物及临床药学研究。
更新日期/Last Update: 2018-04-15