[1]张海林,卢晓君,冯流畅,等.慢性肾脏病湿热型体质易感人群ICAM1基因和IL-10基因启动子多态性研究[J].西部中医药,2018,31(12):1-4.
　ZHANG Hailin,LU Xiaojun,FENG Liuchang,et al.Study on ICAM1 Gene and IL-10 Gene Promoter Polymorphism of Damp-heat Constitution People Susceptible to Chronic Kidney Disease[J].Western Journal of Traditional Chinese Medicine,2018,31(12):1-4.
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慢性肾脏病湿热型体质易感人群ICAM1基因和IL-10基因启动子多态性研究
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
31
期数:
2018年12期
页码:
1-4
栏目:
出版日期:
2018-12-15

文章信息/Info

Title:
Study on ICAM1 Gene and IL-10 Gene Promoter Polymorphism of Damp-heat Constitution People Susceptible to Chronic Kidney Disease
文章编号:
1004-6852(2018)12-0001-04
作者:
张海林1，卢晓君1，冯流畅2，周珊珊1，郑小丰1，王子燕1，韦芳宁3△
1 广州中医药大学第二临床医学院，广东 广州 510405； 2 深圳市龙岗区中医院； 3 广东省中医院
Author(s):
ZHANG Hailin1, LU Xiaojun1, FENG Liuchang2, ZHOU Shanshan1, ZHENG Xiaofeng1, WANG Ziyan1, WEI Fangning3△
1 The Second Clinical Medical School，Guangzhou University of Chinese Medicine，Guangzhou 510405，China； 2 Longgang District TCM Hospital of Shenzhen City；3 Guangdong Provincial Hospital of Traditional Chinese Medicine
关键词:
肾病; 慢性; 湿热体质; ICAM1; 白细胞介素10; 基因多态性
Keywords:
kidney disease; chronic; damp-heat constitution; ICAM1; IL-10; gene polymorphism
分类号:
R962.3
文献标志码:
A
摘要:
目的：探讨岭南地区湿热型体质慢性肾脏病(CKD)1期患者ICAM1基因和白细胞介素10(IL-10)基因启动子的多态性。方法：选择106例CKD1期患者，随机分为对CKD1期湿热质组(50例)、CKD1期平和质组(56例)；选择102例健康体检者，按体质分为健康湿热质组(41例)、健康平和质组(61例)。利用荧光定量PCR方法和ELISA检测方法进行IL-10基因启动子-1082、-819、-592位点多态性研究及ICAM1基因G241和K469位点的多态性分析，并且检测ICAM1、IL-10血清浓度及对比分析。结果：4组IL-10基因启动子、ICAM1基因各位点基因型分布及基因频率比较，差异无统计学意义(P＞0.05)，4组IL-10血清浓度比较差异无统计学意义(P＞0.05)，4组ICAM1血清浓度比较差异有统计学意义(P＜0.05)，且含有G241位点GG基因型血清ICAM1浓度显著比含有GR基因型低，差异有统计学意义(P＜0.05)，含有K469位点不同基因型血清ICAM1浓度比较差异无统计学意义(P＞0.05)。结论：湿热体质人群对CKD具有易感性，ICAM-1 G241位点GG基因型可能是CKD湿热体质预测基因，且通过G241位点GG基因型调控ICAM1，使得ICAM1的表达下调，血清ICAM1浓度降低，减少炎症反应。
Abstract:
Objective：To discuss the promoter polymorphism of ICAM1 gene and IL-10 gene of damp-heat constitution patients suffering chronic kidney disease(CKD) at stage one in Lingnan district. Methods：All 106 CKD1 patients were chosen and randomized into the group of damp-heat constitution CKD1(50 cases) and the group of peaceful constitution CKD1(56 cases); another 102 people who accepted physical examinations were selected and randomized into health group of damp-heat constitution(41 cases) and health group of peaceful constitution(61 cases). Polymorphism of ICAM1 gene G241 and K469 loci were analyzed and polymorphism of IL-10 gene promoters-1082, -819 and -592 loci were studied by fluorescent quantitation PCR methods and ELISA method, the concentrations of ICAM1 and IL-10 were detected, compared and analyzed. Results：The difference had no statistical meaning in the comparisons of genotype distribution and gene frequencies of IL-10 gene promoter and ICAM1 gene(P＞0.05), the difference had no statistical meaning in the comparisons of the concentrations of IL-10 among four groups(P＞0.05), the difference was statistically significant in the comparisons of the concentrations of ICAM1 among four groups(P＜0.05), and the concentrations of ICAM1 in GG genotype serum containing G241 loci were lower than these in GR genotype serum, and the difference had statistical meaning(P＜0.05), the difference had no statistical meaning in the comparisons of the concentrations of ICAM1 in different genotypes serums containing K469 loci(P＞0.05). Conclusion：The people in damp-heat constitution have the susceptibility to CKD, ICAM1-1 G241 loci GG genotype might be the gene predicting damp-heat constitution CKD, and it could regulate ICAM1 by GG genotype G241 loci, reduce the expressions of ICAM1, decrease the concentrations of ICAM1 and relieve inflammatory reaction.

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备注/Memo

备注/Memo:
收稿日期：2018-04-15 *基金项目：广东省科技厅-广东省中医药科学院联合科研专项(编号：财14GKJ02)；广东省清远市科技计划项目(编号2013B002)。 作者简介：张海林(1993—)，女。研究方向：肾病与体质。 △通讯作者：韦芳宁(1961—)，女，博士学位，教授，博士研究生导师，主任医师。研究方向：中医体质学与肾病学结合研究。
更新日期/Last Update: 2018-12-15