[1]任莉莉,问明,季静,等.参附注射液联合细胞因子对人慢性髓性白血病细胞来源树突状细胞诱导生成的影响[J].西部中医药,2014,27(07):4-8.
　REN Lili,WEN Ming,JI Jing,et al.Influence of ShenFu Injection Combined with Cytokines on the Induction and Generation of Dendritic Cells in the Patients with Chronic Myelogenous Leukemia[J].Western Journal of Traditional Chinese Medicine,2014,27(07):4-8.
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参附注射液联合细胞因子对人慢性髓性白血病细胞来源树突状细胞诱导生成的影响
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年07期
页码:
4-8
栏目:
出版日期:
2014-07-15

文章信息/Info

Title:
Influence of ShenFu Injection Combined with Cytokines on the Induction and Generation of Dendritic Cells in the Patients with Chronic Myelogenous Leukemia
文章编号:
1004-6852(2014)07-0004-05
作者:
任莉莉1，问明1，季静1，臧爱民1，宋子正1，徐妍1，李芳1，魏影非2
1 河北大学附属医院，河北 保定 071000； 2 河北医科大学第三医院
Author(s):
REN Lili1, WEN Ming1, JI Jing1, ZANG Aimin1, SONG Zizheng1, XU Yan1, LI Fang1, WEI Yingfei2
1 Affiliated Hospital of Hebei University, Baoding 071000, China; 2 The Third Hospital of Hebei Medical University
关键词:
髓性白血病; 慢性; 细胞因子; 树突状细胞; 诱导分化; 抗肿瘤免疫; 参附注射液
Keywords:
myelogenous leukemia;  chronic;  cytokines;  dendritic cells;  the induction and differentiation;  antitumor immunity;  ShenFu injection
分类号:
R259
摘要:
目的：观察不同浓度参附注射液（SFI）联合细胞因子（GM-CSF/IL-4）对慢性髓性白血病(CML)来源树突状细胞(DC)成熟与功能的影响。方法：采用CML患者的单个核细胞(MNC)，体外诱导扩增DC，在培养体系中分别添加不同浓度的SFI（终浓度分别为：低浓度9.38 mg/mL；中浓度37.50 、75 mg/mL；高浓度150、300 mg/mL生药浓度）、 GM-CSF/IL-4及不同浓度SFI联合细胞因子，通过观察细胞形态、细胞表面分子表达水平、细胞功能的变化，对所获得的细胞进行鉴定。结果：体外单用SFI不能诱导CML细胞分化为DC；细胞因子诱导的CML-DC存在表型及功能障碍；而在含有细胞因子的培养体系中添加SFI，对CML-DC有促进成熟和抑制增殖的双向调节作用，添加高浓度SFI可抑制DC的生长甚至导致细胞死亡，添加中浓度SFI能获得较多成熟CML-DC，可提高其细胞表面共刺激分子的表达水平，并可改善其刺激淋巴细胞增殖的能力及IL-12的含量。结论：适当浓度的SFI可影响CML-DC的分化和成熟，并可改变DC细胞表面免疫分子的表达，增强其功能；SFI最佳诱导浓度为75 mg/mL，且75 mg/mL SFI与GM-CSF/IL-4联合应用有协同作用。
Abstract:
Objective: To observe the effects of ShenFu injection(SFI) in different concentrations combined with cytokines (GM-CSF/IL-4) on the maturation and the function of dendritic cells (DC) in the patients with chronic myelogenous leukemia (CML). Methods: Mononuclear cells were derived from CML patients, and induced into DC in vitro, SFI in different concentrations (ultimate concentrations: low concentration 9.38mg/mL; moderate concentration 37.50 and 75 mg/mL; high concentration 150 and 300 mg/mL crude drug concentration), GM-CSF/IL-4, SFI in different concentrations combined with cytokines were respectively added to culture systems, the obtained cells were identified by observing cellular morphology, the expressions of cellular surface molecules and the changes of cellular functions. Results: CML cells couldn't be induced and differentiated into DC by using SFI in vitro; there was phenotype and functional disorder existed in cytokines-induced CML-DC; while SFI added to the culture system which contained cytokines demonstrated dual-directional regulation to CML-DC: promoting maturation and inhibiting proliferation, high concentrations of SFI could inhibit the growth of DC, even cause cellular death, moderate concentration of SFI could obtain more mature CML-DC, it could improve the expressions of surface costimulatory molecules, the ability of stimulating lymphocytes proliferation and the contents of IL-12. Conclusion: SFI in appropriate density could affects the differentiation and maturation of CML-DC, it could change the expressions of immune molecules of DC cellular surface and enhance its functions, the optimal induction concentration of SFI is 75 mg/mL, SFI at the concentration of 75mg/mL combined with GM-CSF/IL-4 show synergistic action.

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备注/Memo

备注/Memo:
收稿日期：2013-12-27作者简介：任莉莉(1977—)，女，硕士学位，主治医师。研究方向：肿瘤疾病的诊治。
更新日期/Last Update: 2014-07-15