[1]刘其平,强生毅,周钢.吴紫糊中左旋紫草素的含量测定*[J].西部中医药,2014,27(08):14-16.
　LIU Qiping,QIANG Shengyi,ZHOU Gang.Content Determination of Shikonin in WuZi Cataplasm[J].Western Journal of Traditional Chinese Medicine,2014,27(08):14-16.
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吴紫糊中左旋紫草素的含量测定*
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
27
期数:
2014年08期
页码:
14-16
栏目:
出版日期:
2014-08-15

文章信息/Info

Title:
Content Determination of Shikonin in WuZi Cataplasm
文章编号:
1004-6852(2014)08-0014-03
作者:
刘其平，强生毅，周钢
白银市第二人民医院，甘肃 白银 730900
Author(s):
LIU Qiping, QIANG Shengyi, ZHOU Gang
Baiyin Municipality the Second People′s Hospital, Baiyin 730900, China
关键词:
吴紫糊; 高效液相色谱法; 左旋紫草素; 含量测定
Keywords:
WuZi cataplasm;  high performance liquid chromatography;  shikonin;  content determination
分类号:
R927.2
文献标志码:
A
摘要:
目的：建立吴紫糊中左旋紫草素的测定方法。方法：高效液相色谱法，色谱条件色谱柱为安捷伦C18柱(4.6 mm×250 mm，5 μm)，流动相为甲醇-0.1%磷酸盐缓冲液(80∶20)，检测波长516 nm，柱温25℃，流速10 mL/min。结果：进样量在8.2～41.0 μg/mL范围内，线性关系良好(r=0.999 9)平均回收率为101.30％(RSD为1.55％，n=9)。结论：高效液相色谱法测定吴紫糊中左旋紫草素含量结果准确、重现性好，适用于吴紫糊的质量控制。
Abstract:
Objective: To establish the determination method for shikonin in WuZi cataplasm. Methods: High performance liquid chromatography(HPLC), chromatographic condition, chromatographic column: agilent C18 column (4.6 mm×250 mm, 5 μm), mobile phase: methanol-0.1% phosphate buffer solution(80:20), detection wavelength: 516nm, column temperature:25℃, flow rate was 10mL/min. Results: Sample size was in the range between 8.2 and 41.0 μg/mL, linear relationship was better (r=0.999 9), average recovery rate was 101.30% (RSD was 1.55%, n=9). Conclusion: HPLC determining the contents of shikonin in WuZi cataplasm is accurate and reproducible, which could be applied to quality control of WuZi cataplasm.

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备注/Memo

备注/Memo:
收稿日期：2014-03-05*基金项目：白银市2010年第二批科技计划项目（编号 GK20103-2-059A）作者简介：刘其平(1974—)，男，主管药师。研究方向：中药制剂的研究。
更新日期/Last Update: 2014-08-15