[1]何芬莉,李康乐,尚荣国,等.高效液相色谱法同时测定康痔栓中黄芩苷和蒙花苷两种成分[J].西部中医药,2020,33(03):42-44.[doi:10.12174/j.issn.1004-6852.2020.03.11]
　HE Fenli,LI Kangle,SHANG Rongguo,et al.Determination of Baicalin and Linarin of Kangzhi Suppository by HPLC Simultaneously[J].Western Journal of Traditional Chinese Medicine,2020,33(03):42-44.[doi:10.12174/j.issn.1004-6852.2020.03.11]
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高效液相色谱法同时测定康痔栓中黄芩苷和蒙花苷两种成分
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年03期
页码:
42-44
栏目:
出版日期:
2020-03-15

文章信息/Info

Title:
Determination of Baicalin and Linarin of Kangzhi Suppository by HPLC Simultaneously
文章编号:
1004-6852(2020)03-0042-03
作者:
何芬莉，李康乐，尚荣国，杨小娟，叶凯，窦建卫
1 蒲城县食品药品监督管理局，陕西 渭南 714000； 2 西安交通大学药学院；3 甘肃中医药大学基础医学院
Author(s):
HE Fenli1, LI Kangle2, SHANG Rongguo2, YANG Xiaojuan3, YE Kai 3, DOU Jianwei 2△
1 Pucheng County Food and Drug Administration, Weinan 714000, China; 2 College of Pharmacy, Xi′an Jiaotong University; 3 College of Basic Medical Sciences, Gansu University of Traditional Chinese Medicine
关键词:
康痔栓; 黄芩苷; 蒙花苷; 高效液相色谱法
Keywords:
Kangzhi suppository;  baicalin;  linarin;  HPLC
分类号:
R284
DOI:
10.12174/j.issn.1004-6852.2020.03.11
摘要:
目的：探讨高效液相色谱法(high performance liquid chromatography，HPLC)同时测定康痔栓中黄芩苷和蒙花苷两种有效成分的方法。方法：使用Zorbax Rx-C18(4.6 mm×250 mm×5 μm)色谱柱，以甲醇-0.3%磷酸水溶液为流动相，梯度洗脱，流动相流速为1 mL/min，检测波长分别为280 nm和334 nm。结果：康痔栓中黄芩苷和蒙花苷两种有效成分的峰面积与其进样量有较好的线性关系，相关系数均超过0.999 5，仪器精密度标准差不超过2%，平均回收率均大于96%。结论：采用HPLC同时测定康痔栓中黄芩苷和蒙花苷两种有效成分，快速、便捷、回收率高，是测定康痔栓中两种标志性有效成分的有效方法。
Abstract:
Objective: To explore the method of detecting baicalin and linarin of Kangzhi suppository by HPLC simultaneously. Methods: Zorbax Rx - C18(4.6 mm×250 mm×5 μm) chromatographic column was used in the study, methanol - 0.3% phosphoric acid aqueous solution as mobile phase, gradient elution, the flow rate 1mL/min, detection wavelength were 280nm and 334nm. Results: Peak area of two active ingredients baicalin and linarin of Kangzhi suppository show better linear relationship with their sample size, and the correlation coefficients are more than 0.999 5, the precision standard derivation of the instrument is not more than 2%, average recovery rate was greater than 96%. Conclusion: Detecting two active ingredients baicalin and linarin of Kangzhi suppository by HPLC simultaneously, which is rapid, convenient with high recovery, is the effective method of detecting two active ingredients in Kangzhi suppository.

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备注/Memo

备注/Memo:
收稿日期：2019-03-27*基金项目：国家自然科学基金项目(30300444)；必康制药科研基金(2017bikangjijin-001)。作者简介：何芬莉(1976—)，女，主管药师。研究方向：药品检验与质量控制研究。△通讯作者：窦建卫(1968—)，男，博士学位，副教授，副主任医师。研究方向：中药复方药效物质基础研究。
更新日期/Last Update: 2020-03-15