[1]李会婷,史天陆△,付恩桃,等.RP-HPLC法同时测定咽炎片中黄芩苷和丹皮酚的含量[J].西部中医药,2020,33(01):56.[doi:10.12174/j.issn.1004-6852.2020.01.15]
　LI Huiting,SHI Tianlu,FU Entao,et al.Determination of Baicalin and Paeonol Contained in Pharyngitis Tablets by RP-HPLC Simultaneously[J].Western Journal of Traditional Chinese Medicine,2020,33(01):56.[doi:10.12174/j.issn.1004-6852.2020.01.15]
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RP-HPLC法同时测定咽炎片中黄芩苷和丹皮酚的含量
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《西部中医药》[ISSN:2096-9600/CN:62-1204/R]

卷:
33
期数:
2020年01期
页码:
56
栏目:
出版日期:
2020-01-15

文章信息/Info

Title:
Determination of Baicalin and Paeonol Contained in Pharyngitis Tablets by RP-HPLC Simultaneously
文章编号:
1004-6852(2020)01-0056-04
作者:
李会婷1，史天陆1△，付恩桃2，胥振国2
1 中国科学技术大学附属第一医院/安徽省立医院药剂科，安徽 合肥 230001； 2 合肥职业技术学院生物工程学院
Author(s):
LI Huiting1, SHI Tianlu1△, FU Entao2, XU Zhenguo2
1 Department of Pharmacy, Anhui Provincial Hospital/The First Affiliated Hospital of USTC, Hefei 230001, China; 2 College of Biological Engineering, Hefei College of Vocational Technology
关键词:
咽炎片; 黄芩苷; 丹皮酚; 高效液相色谱法
Keywords:
pharyngitis tablets;  baicalin;  paeonol;  HPLC
分类号:
R927.2
DOI:
10.12174/j.issn.1004-6852.2020.01.15
文献标志码:
A
摘要:
目的：建立同时测定咽炎片中黄芩苷和丹皮酚含量的高效液相色谱法。方法：采用C18色谱柱(4.6 mm×250 mm，5 μm)，甲醇-0.1%磷酸水溶液(47∶53)，检测波长278 nm，柱温30 ℃，流速1.0 mL/min。结果：黄芩苷在0.51～20.40 μg/mL范围内，丹皮酚在0.248～9.90 μg/mL范围内线性关系良好。平均回收率(n=9)分别为99.2%，99.8%，含量测定结果表明，不同生产企业黄芩苷和丹皮酚含量差异较大。结论：经方法学验证，该法可用于咽炎片中黄芩苷和丹皮酚含量的快速测定。
Abstract:
Objective: To establish a HPLC method of determining baicalin and paeonol in pharyngitis tablets simultaneously. Methods: C18 chromatographic column(4.6 mm × 250 mm, 5 μm) was used with methanol-0.1% phosphoric acid solution(47:53), the detection wavelength was 278 nm, the column temperature was 30℃ and the flow rate was 1.0 mL/min. Results: Baicalin in the range between 0.51 and 20.40 μg/mL and paeonol in the range between 0.248 and 9.90 μg/mL presented good linear relationship. Average recovery rates (n=9) were 99.2% and 99.8% respectively, the results of content determination showed that greater differences existed in the contents of baicalin and paeonol from different manufacturing enterprises. Conclusion: Tested by the methodolgy, the method could be used for rapid determination of the contents of baicalin and paeonol in pharyngitis tablets.

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备注/Memo

备注/Memo:
收稿日期：2018-12-20*基金项目：2017年安徽省自然科学基金(1708085MH225)；安徽省名师(大师)工作室项目(2016msgzs062)；合肥职业技术学院校级自然科学重点项目(201714KJA005)。作者简介：李会婷(1990—)，女，硕士学位，药师。研究方向：临床药学与药物质量评价。△通讯作者：史天陆(1975—)，男，博士学位，主任药师。研究方向：药物利用评价。
更新日期/Last Update: 2020-01-15